<i>In planta</i> particle bombardment (iPB): A new method for plant transformation and genome editing
نویسندگان
چکیده
منابع مشابه
Stable transformation of plant cells by particle bombardment/biolistics.
Particle bombardment, or biolistics, is a commonly used method for genetic transformation of plants and other organisms. Millions of DNA-coated metal particles are shot at target cells or tissues using a biolistic device or gene gun. The DNA elutes off the particles that lodge inside the cells, and a portion may be stably incorporated in the host chromosomes. A protocol for the generation of tr...
متن کاملStable Transformation of Saintpaulia ionantha by Particle Bombardment
Background: A highly efficient genetic transformation system is essential for a successful genetic manipulation of the African violet (Saintpaulia ionantha Wendl.). Objectives: Developing a particle bombardment-based genetic transformation system for the African violet. Materials and Methods: A local cultivar of the African violet from Guilan province was used for transformation experiments. Th...
متن کاملIs Genome Editing Techniques the New Challenge for Plant Breeding?
Plant breeding is the “art and science” of the genetic improvement of crops to produce new varieties that had a large impact on crop yield and food production. Genetic variation is the engine that propels breeding to meet future challenges and this variation is due to intraand inter-specific recombination. The recombinant DNA technology marked the beginning of a new era, indeed conventional pla...
متن کاملstable transformation of saintpaulia ionantha by particle bombardment
background: a highly efficient genetic transformation system is essential for a successful genetic manipulation of the african violet (saintpaulia ionantha wendl.).objectives: developing a particle bombardment-based genetic transformation system for the african violet.materials and methods: a local cultivar of the african violet from guilan province was used for transformation experiments. the ...
متن کاملGenetic transformation of silver birch (Betula pendula) by particle bombardment.
We used in vitro callus and shoot cultures as target material for genetic transformation of silver birch (Betula pendula Roth) by particle bombardment. Cultivation of in vitro shoot cultures before particle bombardment and a long selection period, combined with a high concentration of selective agent after bombardment, led to the production of transformed plantlets that were stable, and no esca...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2020
ISSN: 1342-4580,1347-6114
DOI: 10.5511/plantbiotechnology.20.0206a